Journal: Drug Design, Development and Therapy

Article Title:

miR-370-3p Alleviates Ulcerative Colitis-Related Colorectal Cancer in Mice Through Inhibiting the Inflammatory Response and Epithelial-Mesenchymal Transition

doi: 10.2147/dddt.s238124

Figure Lengend Snippet: Figure 3 Effect of miR-370-3p on inflammatory response and TLR4 signaling pathway in AOM/DSS-induced mice. (A) The expression of TNF-α, IL-1β, and IL-6 in colonic tissues of mice from the Sham group, UC-CRC group, Ad-control group, and Ad-miR-370-3p group was detected by qRT-PCR. (B) The production of TNF-α and PGE2 in the lysate of the tumor colon tissues were detected by ELISA. (C) The expression of TLR4 and COX-2 in the tumor colon tissues from each group was evaluated by using IHC staining. Scale bar=50 μm. (D) The expression levels of TLR4, COX-2, and pEGFR in the tumor colon tissues from each group were determined by Western blot, and the relative band intensity was analyzed.The comparison among groups was performed by One-way ANOVA. Data are presented as mean ± SD. ##p<0.01 compared with the Sham group, **p<0.01 compared with the Ad-control group.

Article Snippet: After blocking with 5% skim milk (Sangon Biotech, Shanghai China) at room temperature for 1 h, the membranes were incubated with corresponding primary antibodies incuding TLR4 (1:1000, proteintech, Wuhan, China), COX-2 (1:1000, proteintech, Wuhan, China), pEGFR (phosphorylated epidermal growth factor receptor, 1:1000, Affinity, Changzhou, Jiangsu, China), β-catenin (1:5000, proteintech, Wuhan, China), p53 (1:3000, proteintech, Wuhan, China), ki67 (1:1000, Affinity, Changzhou, Jiangsu, China), E-cadherin (1:10,000, proteintech, Wuhan, China), N-cadherin (1:5000, proteintech, Wuhan, China), Vimentin (1:5000, proteintech, Wuhan, China) and GAPDH (1:10,000, proteintech, Wuhan, China) overnight at 4° C. Then the membranes were washed by TBST and incubated with appropriate secondary HRPconjugated goat anti-rabbit or goat anti-mouse antibodies (1:3000, Solarbio, Beijing, China) for 1 h at 37 ° C. The proteins were visualized by using ECL Western Blotting Substrate (Solarbio, Beijing, China).

Techniques: Expressing, Control, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Western Blot, Comparison

Journal: Molecules

Article Title: Pedicularis resupinata Extract Prevents Depressive-like Behavior in Repeated Corticosterone-Induced Depression in Mice: A Preliminary Study

doi: 10.3390/molecules27113434

Figure Lengend Snippet: Effect of PRE on glucocorticoid receptor activity in CORT-induced depressive mice. Hippocampal Western blot analysis revealed that CORT injections significantly decreased BDNF ( a ) expression and increased tGR ( b ) and Ser211 ( c ) expression in the hippocampus, while treatment with PRE at 300 mg/kg significantly attenuated the effect. Results are presented as mean ± SEM ( n = 4, per group). # p < 0.05, ## p < 0.01, and ### p < 0.001 versus the sham group; * p < 0.05, and ** p < 0.01 versus the CORT-injected Con group. Con, control; CORT, corticosterone; St. JW, St. John’s wort extract; PRE, P. resupinata extract.

Article Snippet: The membranes were blocked with 4% skim milk in Tris-buffered saline with 1% Tween-20 for 40 min, and subsequently probed with the following primary antibodies: BDNF mouse monoclonal antibody (1:1000 dilution, sc-65514, Santa Cruz Biotechnology, CA, USA), glucocorticoid receptor (1:1000 dilution, sc-393232, Santa Cruz Biotechnology, CA, USA; GR phosphorylation at serine 211 (Ser211, 1:1000 dilution, #4161, Cell Signaling, MA, USA), and anti-β-actin rabbit polyclonal antibody (1:1000 dilution, #4967, Cell Signaling, MA, USA) overnight at 4 °C in 3% skim milk in TBST.

Techniques: Activity Assay, Western Blot, Expressing, Injection, Control

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Hypocrellin A exerts antitumor effects by inhibiting the FGFR1 signaling pathway in non-small cell lung cancer.

doi: 10.1016/j.phymed.2022.153924

Figure Lengend Snippet: Fig. 4. HA inhibited NSCLC activity through the FGFR1 signaling pathway. (A) Molecular docking model of HA with FGFR1. (B) Experimental confirmation of the FGFR1–HA interaction. The FGFR1 protein captured on and NTA chip could bind HA, with an affinity constant of 3.92 μM, as determined by an SPR assay. (C) After treating H460, PC-9, and H1975 cells with different concentrations of HA for 24 h, Western blot analysis was used to analyze the phosphorylation of FGFR1, MAPK, and AKT. Unpaired t tests were performed to analyze relative expression and phosphorylation of proteins in the FGFR1 pathway in the treatment group vs control group according to quantified results of Western blot analyses. GAPDH served as an internal reference. *p< 0.05, **p< 0.01 versus DMSO, n = 3.

Article Snippet: The antibodies against phosphorylated FGFR1 (P-FGFR1), Survivin, MCL-1, STAT3, cleaved poly(ADP-ribose) polymerase (Cl-PARP), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology (MA, USA).

Techniques: Activity Assay, SPR Assay, Western Blot, Phospho-proteomics, Expressing, Control

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Hypocrellin A exerts antitumor effects by inhibiting the FGFR1 signaling pathway in non-small cell lung cancer.

doi: 10.1016/j.phymed.2022.153924

Figure Lengend Snippet: Fig. 5. HA inhibited the STAT3 signaling pathway by inhibiting FGFR1. After treating H460, PC-9, and H1975 cells with different concentrations of HA for 24 h, western blot analysis was used to analyze the phosphorylation level of STAT3 and expression of MCL-1 and Survivin. The unpaired t-test statistics of the relative expression of the STAT3 pathway in the experimental group/control group of the Western blot analysis are presented. GAPDH served as an internal reference. *p< 0.05, **p< 0.01 versus DMSO, n = 3.

Article Snippet: The antibodies against phosphorylated FGFR1 (P-FGFR1), Survivin, MCL-1, STAT3, cleaved poly(ADP-ribose) polymerase (Cl-PARP), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology (MA, USA).

Techniques: Western Blot, Phospho-proteomics, Expressing, Control

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Hypocrellin A exerts antitumor effects by inhibiting the FGFR1 signaling pathway in non-small cell lung cancer.

doi: 10.1016/j.phymed.2022.153924

Figure Lengend Snippet: Fig. 6. HA inhibited the progression of NSCLCin vivo. (A) HA (2.5 mg/kg or 5 mg/kg), AZD4547 (5 mg/kg), and the control group (treated with an equivalent volume of PBS); changes in the tumor volume in mice after treatment in NSCLC xenograft tumor models. (B) Tumor weight of mice after seven injections of HA. (C) Image of mouse tumor after seven injections of HA. (D) Weight of the mouse every other day. (E) Representative pictures of the hematoxylin and eosin staining of mouse tumors. (F) Analysis of the expression level of the target protein in mouse tumors by the Western blot analysis. Below the band is the unpaired t-test statistics chart of the relative expression of FGFR1 and molecules in the STAT3 pathway in the treatment group and control group from the western blot analysis. GAPDH served as an internal reference. *p< 0.05, **p< 0.01 versus Conrtol, n = 5.

Article Snippet: The antibodies against phosphorylated FGFR1 (P-FGFR1), Survivin, MCL-1, STAT3, cleaved poly(ADP-ribose) polymerase (Cl-PARP), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology (MA, USA).

Techniques: Control, Staining, Expressing, Western Blot

Journal: Molecules

Article Title: Pedicularis resupinata Extract Prevents Depressive-like Behavior in Repeated Corticosterone-Induced Depression in Mice: A Preliminary Study

doi: 10.3390/molecules27113434

Figure Lengend Snippet: Effect of PRE on glucocorticoid receptor activity in CORT-induced depressive mice. Hippocampal Western blot analysis revealed that CORT injections significantly decreased BDNF ( a ) expression and increased tGR ( b ) and Ser211 ( c ) expression in the hippocampus, while treatment with PRE at 300 mg/kg significantly attenuated the effect. Results are presented as mean ± SEM ( n = 4, per group). # p < 0.05, ## p < 0.01, and ### p < 0.001 versus the sham group; * p < 0.05, and ** p < 0.01 versus the CORT-injected Con group. Con, control; CORT, corticosterone; St. JW, St. John’s wort extract; PRE, P. resupinata extract.

Article Snippet: The membranes were blocked with 4% skim milk in Tris-buffered saline with 1% Tween-20 for 40 min, and subsequently probed with the following primary antibodies: BDNF mouse monoclonal antibody (1:1000 dilution, sc-65514, Santa Cruz Biotechnology, CA, USA), glucocorticoid receptor (1:1000 dilution, sc-393232, Santa Cruz Biotechnology, CA, USA; GR phosphorylation at serine 211 (Ser211, 1:1000 dilution, #4161, Cell Signaling, MA, USA), and anti-β-actin rabbit polyclonal antibody (1:1000 dilution, #4967, Cell Signaling, MA, USA) overnight at 4 °C in 3% skim milk in TBST.

Techniques: Activity Assay, Western Blot, Expressing, Injection, Control